EV71 VP1 Recombinant Protein
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Specification
Product Description
EV71 VP1 partial ORF ( AAP82198.1, 1 a.a. - 297 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
GDRVADVIESSIGDSVSRALTQALPAPTGQNTQVSSHRLDTGEVPALQAAEIGASSNTSDESMIETRCVLNSHSTAETTLDSFFSRAGLVGEIDLPLEGTTNPNGYANWDIDITGYAQMRRKVELFTYMRFDAEFTFVACTPTGGVVPQLLQYMFVPPGAPKPDSRESLAWQTATNPSVFVKLTDPPAQVSVPFMSPASAYQWFYDGYPTFGEHKQEKDLEYGACPNNMMGTFSVRTVGSSKSKYPLVVRIYMRMKHVRAWIPRPMRNQNYLFKANPNYAGNSIKPTGTSRTAITTL
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
59.07
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Publication Reference
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Inhibitory effects and mechanisms of proanthocyanidins against enterovirus 71 infectio.
Jiqin Sun, Xiaoyao Ma, Lishan Sun, Yang Zhang, Cui Hao, Wei Wang.
Virus Research 2023 May; 329:199098.
Application:Sub, Recombinant proteins.
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VP1-binding protein glucose-regulated protein 78 as an important mediator for enterovirus 71 infecting human brain microvascular endothelial cells.
Luo W, Liang P, Puthiyakunnon S, Yang L, Hong C.
Indian Journal of Medical Microbiology 2020 Jul; 37(3):406.
Application:Func, PI, WB-Re, Human, Human brain microvascular endothelial cells.
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The C-Terminus of the Core β-Ladder domain in Japanese Encephalitis Virus Nonstructural Protein 1 Is Flexible for Accommodation of Heterologous Epitope Fusion.
Yen LC, Liao JT, Lee HJ, Chou WY, Chen CW, Lin YL, Liao CL.
Journal of Virology 2015 Nov; 90(3):1178.
Application:WB, Mouse, Serum.
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Human IgG Fc promotes expression, secretion and immunogenicity of enterovirus 71 VP1 protein.
Xu J, Zhang C.
Journal of Biomedical Research 2016 May; 30(3):209.
Application:ELISA, Mouse, Serum.
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Glycyrrhizic acid as the antiviral component of Glycyrrhiza uralensis Fisch. against coxsackievirus A16 and enterovirus 71 of hand foot and mouth disease.
Wang J, Chen X, Wang W, Zhang Y, Yang Z, Jin Y, Hui Ming G, Li E, Yang G.
Journal of Ethnopharmacology 2013 May; 147(1):114.
Application:WB, Monkey, Vero cells.
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Identification of OASL d, a splice variant of human OASL, with antiviral activity.
Guo X, Li X, Xu Y, Sun T, Yang G, Wu Z, Li E.
The International Journal of Biochemistry & Cell Biology 2012 Jul; 44(7):1133.
Application:WB, Human, RD cells.
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Inhibitory effects and mechanisms of proanthocyanidins against enterovirus 71 infectio.
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