Ahsa1 monoclonal antibody, clone 25F2.D9
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot using Ahsa1 monoclonal antibody, clone 25F2.D9 (Cat # MAB8169) shows detection of a band ~42 KDa in size corresponding to Ahsa1 in A-431 whole cell lysate (Lane 1) and MCF-7 whole cell lysate (Lane 2). A control lane is shown where primary ahntibody was omitted from the incubation (Lane 3). Molecular weight markers are shown at the left. For best results, block the membrane overnight with 3% BSA in TBS followed by reaction with primary antibody diluted 1 : 1,000 and use HRP conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer for detection.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of Ahsa1 monoclonal antibody, clone 25F2.D9 (Cat # MAB8169) was used at a 5-10 ug/mL to detect Ahsa1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. Expression of Ahsa1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.
-
Specification
Product Description
Rat monoclonal antibody raised against full length recombinant Ahsa1.
Immunogen
Recombinant protein corresponding to full length mouse Ahsa1.
Host
Rat
Reactivity
Chimpanzee, Human, Mouse
Form
Liquid
Recommend Usage
ELISA (1:20000)
Western Blot (1:1000)
Immunohistochemistry (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot using Ahsa1 monoclonal antibody, clone 25F2.D9 (Cat # MAB8169) shows detection of a band ~42 KDa in size corresponding to Ahsa1 in A-431 whole cell lysate (Lane 1) and MCF-7 whole cell lysate (Lane 2). A control lane is shown where primary ahntibody was omitted from the incubation (Lane 3). Molecular weight markers are shown at the left. For best results, block the membrane overnight with 3% BSA in TBS followed by reaction with primary antibody diluted 1 : 1,000 and use HRP conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer for detection.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of Ahsa1 monoclonal antibody, clone 25F2.D9 (Cat # MAB8169) was used at a 5-10 ug/mL to detect Ahsa1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. Expression of Ahsa1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — Ahsa1
-
Publication Reference
-
The charged linker region is an important regulator of Hsp90 function.
Hainzl O, Lapina MC, Buchner J, Richter K.
The Journal of Biological Chemistry 2009 Jun; 284(34):22559.
-
A novel HSP90 chaperone complex regulates intracellular vesicle transport.
Lotz GP, Brychzy A, Heinz S, Obermann WM.
Journal of Cell Science 2008 Feb; 121(Pt 5):717.
-
Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis.
Wang X, Venable J, LaPointe P, Hutt DM, Koulov AV, Coppinger J, Gurkan C, Kellner W, Matteson J, Plutner H, Riordan JR, Kelly JW, Yates JR 3rd, Balch WE.
Cell 2006 Nov; 127(4):803.
Application：WB, Human, HBE, HEK 293 cells.
-
The charged linker region is an important regulator of Hsp90 function.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com