OLLAS tag monoclonal antibody, clone L2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot of comparison of binding sensitivity of OLLAS tag monoclonal antibody, clone L2 (Cat # MAB7372), DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Western Blot (Recombinant protein)
Western blot of comparison of binding sensitivity of OLLAS tag monoclonal antibody, clone L2 (Cat # MAB7372), DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
-
Specification
Product Description
Rat monoclonal antibody raised against OLLAS tag.
Immunogen
A synthetic peptide corresponding to OLLAS -tagged extracellular domain of mouse Langerin.
Sequence
SGFANELGPRLMGK
Host
Rat
Reactivity
Human, Mouse, Rat
Specificity
OLLAS Epitope Tag (L2).
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Immunofluorescence (1:10)
Western Blot (1:1000)
Immunocytochemistry (1:10-1:100)
Immunohistochemistry (Frozen sections) (1:10-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot of comparison of binding sensitivity of OLLAS tag monoclonal antibody, clone L2 (Cat # MAB7372), DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.Western Blot (Recombinant protein)
Western blot of comparison of binding sensitivity of OLLAS tag monoclonal antibody, clone L2 (Cat # MAB7372), DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
-
Publication Reference
-
DamID transcriptional profiling identifies the Snail/Scratch transcription factor Kahuli as an Alk target in the Drosophila visceral mesoderm.
Patricia Mendoza-Garcia, Swaraj Basu, Sanjay Kumar Sukumar, Badrul Arefin, Georg Wolfstetter, Vimala Anthonydhason, Linnea Molander, Ezgi Uçkun, Henrik Lindehell, Cristina Lebrero-Fernandez, Jan Larsson, Erik Larsson, Mats Bemark, Ruth H Palmer.
Development 2021 Dec; 148(23):0.
Application：IHC, Drosophila, Visceral and somatic mesoderm.
-
Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs.
Yamazaki Y, Palmer L, Alexandre C, Kakugawa S, Beckett K, Gaugue I, Palmer RH, Vincent JP.
Amino Acids 2016 Mar; 18(4):451.
Application：IF, Fruit fly, Wingless along the apico-basal axis from Fruit fly.
-
Drosophila S2 cells secrete wingless on exosome-like vesicles but the wingless gradient forms independently of exosomes.
Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Le Borgne R, Vincent JP.
Traffic 2013 Jan; 14(1):82.
Application：IF, Fruit fly, Wing disc.
-
Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo.
Idoyaga J, Cheong C, Suda K, Suda N, Kim JY, Lee H, Park CG, Steinman RM.
Journal of Immunology 2008 Mar; 180(6):3647.
Application：Flow Cyt, WB, Mouse, Mouse dendritic cells.
-
Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection.
Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG.
Journal of Immunological Methods 2008 Feb; 331(1-2):27.
Application：IF, IP, WB-Tr, Human, 293T cells.
-
DamID transcriptional profiling identifies the Snail/Scratch transcription factor Kahuli as an Alk target in the Drosophila visceral mesoderm.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com