ENTPD1 monoclonal antibody, clone BU61
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More Files
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Flow Cytometry
5 X 105 ficoll prepared human peripheral blood lymphocytes were washed and incubated 45 minutes on ice with 80 ml of anti-CD39 (FITC) at a 1 : 50 dilution. Cells were washed three times, fixed and analyzed on a BD FACstar plus. A net 2.2% population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was 100% blocked when cells were pre incubated 10 minutes with 20 ml of 0.5 mg/mL ENTPD1 monoclonal antibody, clone BU61 (Cat # MAB6910).
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Specification
Product Description
Mouse monoclonal antibody raised against native ENTPD1.
Immunogen
Native purified human WM-1 (Waldenstrom's macroglobulinemia) cell line.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
5 X 105 ficoll prepared human peripheral blood lymphocytes were washed and incubated 45 minutes on ice with 80 ml of anti-CD39 (FITC) at a 1 : 50 dilution. Cells were washed three times, fixed and analyzed on a BD FACstar plus. A net 2.2% population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was 100% blocked when cells were pre incubated 10 minutes with 20 ml of 0.5 mg/mL ENTPD1 monoclonal antibody, clone BU61 (Cat # MAB6910). -
Gene Info — ENTPD1
Entrez GeneID
953Gene Name
ENTPD1
Gene Alias
ATPDase, CD39, DKFZp686D194, DKFZp686I093, FLJ40921, FLJ40959, NTPDase-1
Gene Description
ectonucleoside triphosphate diphosphohydrolase 1
Omim ID
601752Gene Ontology
HyperlinkOther Designations
CD39 antigen|OTTHUMP00000020161|ecto-ATP diphosphohydrolase|ecto-apyrase|lymphoid cell activation antigen
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Interactome
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Pathway
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Disease
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Publication Reference
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The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterization.
Maliszewski CR, Delespesse GJ, Schoenborn MA, Armitage RJ, Fanslow WC, Nakajima T, Baker E, Sutherland GR, Poindexter K, Birks C, et al..
The Journal of Immunology 1994 Oct; 153(8):3574.
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Cloning and sequencing of a trophoblast-endothelial-activated lymphocyte surface protein: cDNA sequence and genomic structure.
Voland JR, Wyzykowski RJ, Huang M, Dutton RW.
PNAS 1992 Nov; 89(21):10425.
Application：Flow Cyt, IP, Human, Monkey, COS cells, Human trophoblasts.
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The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterization.
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