Ubinuclein 1 monoclonal antibody, clone UBN1-02
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More Files
- More Functions
Western Blot (Cell lysate)
Western blotting analysis of Ubinuclein 1 in nuclear fraction of HeLa cells. The Ubinuclein 1 monoclonal antibody, clone UBN1-02 (Cat # MAB6459) (lane 1, 2) detects ubinuclein 1. Antibody dilution : Lane 1 - 1 ug/mL ; Lane 2 - 5 ug/mL .
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Ubinuclein 1.
Immunogen
Recombinant protein corresponding to amino acids 1-190 of Ubinuclein 1.
Host
Mouse
Theoretical MW (kDa)
165
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Immunoprecipitation
Western Blot (1-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blotting analysis of Ubinuclein 1 in nuclear fraction of HeLa cells. The Ubinuclein 1 monoclonal antibody, clone UBN1-02 (Cat # MAB6459) (lane 1, 2) detects ubinuclein 1. Antibody dilution : Lane 1 - 1 ug/mL ; Lane 2 - 5 ug/mL .Immunoprecipitation
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Publication Reference
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Characterization of the ubinuclein protein as a new member of the nuclear and adhesion complex components (NACos).
Aho S, Lupo J, Coly PA, Sabine A, Castellazzi M, Morand P, Sergeant A, Manet E, Boyer V, Gruffat H.
Biology of the Cell 2009 Jun; 101(6):319.
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Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells.
Banumathy G, Somaiah N, Zhang R, Tang Y, Hoffmann J, Andrake M, Ceulemans H, Schultz D, Marmorstein R, Adams PD.
Molecular and Cellular Biology 2009 Feb; 29(3):758.
Application：IF, WB-Ce, Human, IMR90, WI-38 cells.
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Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors.
Aho S, Buisson M, Pajunen T, Ryoo YW, Giot JF, Gruffat H, Sergeant A, Uitto J.
The Journal of Cell Biology 2000 Mar; 148(6):1165.
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Characterization of the ubinuclein protein as a new member of the nuclear and adhesion complex components (NACos).
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