CD14 monoclonal antibody, clone biG 2 (RoMo-1)
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Specification
Product Description
Mouse monoclonal antibody raised against native CD14.
Immunogen
Native from human monocytes.
Host
Mouse
Reactivity
Human
Specificity
biG 2 (RoMo-1) represents an excellent marker for CD14. CD14 has been shown to be involved in the LPS-induced cell activation. This antigen is present as a membrane bound form and as soluble antigen, both involved in cell stimulation by LPS. The plasma protein LBP plays an important role in this LPS-CD14 mediated cell activation. Monocytes and PMN are activated by the LBP-LPS complex via membrane CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.
Form
Lyophilized
Isotype
IgG2a
Recommend Usage
Flow Cytometry (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — CD14
Entrez GeneID
929Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Pathway
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Disease
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Publication Reference
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LPS-binding protein and CD14-dependent attachment of hepatitis B surface antigen to monocytes is determined by the phospholipid moiety of the particles.
Vanlandschoot P, Van Houtte F, Roobrouck A, Farhoudi A, Stelter F, Peterson DL, Gomez-Gutierrez J, Gavilanes F, Leroux-Roels G.
The Journal of General Virology 2002 Sep; 83(Pt 9):2279.
Application:Flow Cyt, Func, Human, PBMCs.
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Binding of bacterial peptidoglycan to CD14.
Dziarski R, Tapping RI, Tobias PS.
The Journal of Biological Chemistry 1998 Apr; 273(15):8680.
Application:Added, Recombinant protein.
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LPS-binding protein and CD14-dependent attachment of hepatitis B surface antigen to monocytes is determined by the phospholipid moiety of the particles.
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