LITAF monoclonal antibody, clone AT1F9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate.
Immunofluorescence
Immunofluorescence analysis of LnCap cells. The cell was stained with LITAF monoclonal antibody, clone AT1F9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant LITAF.
Immunogen
Recombinant protein corresponding to full length human LITAF.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of LnCap cells. The cell was stained with LITAF monoclonal antibody, clone AT1F9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — LITAF
Entrez GeneID
9516Protein Accession#
NP_004853Gene Name
LITAF
Gene Alias
FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276, PIG7, SIMPLE, TP53I7
Gene Description
lipopolysaccharide-induced TNF factor
Gene Ontology
HyperlinkGene Summary
Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppresor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
LPS-induced TNF-alpha factor|lipopolysaccharide-induced TNF-alpha factor|lipopolysaccharide-induced tumor necrosis factor-alpha factor|p53-induced gene 7 protein|small integral membrane protein of lysosome/late endosome|tumor protein p53 inducible protein
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Interactome
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Disease
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Publication Reference
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Litaf/Simple protein is increased in intestinal tissues from patients with CD and UC, but is unlikely to function as a transcription factor.
Huang Y, Bennett CL.
Inflammatory Bowel Diseases 2007 Jan; 13(1):120.
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LPS induces the interaction of a transcription factor, LPS-induced TNF-alpha factor, and STAT6(B) with effects on multiple cytokines.
Tang X, Marciano DL, Leeman SE, Amar S.
PNAS 2005 Apr; 102(14):5132.
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Identification and functional characterization of a novel binding site on TNF-alpha promoter.
Tang X, Fenton MJ, Amar S.
PNAS 2003 Apr; 100(7):4096.
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Litaf/Simple protein is increased in intestinal tissues from patients with CD and UC, but is unlikely to function as a transcription factor.
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