Ngfr monoclonal antibody, clone MC192
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining in rat forebrain using Ngfr monoclonal antibody, clone MC192 (Cat # MAB5595). Paraformaldehyde fixed rat brain sections were incubated with 1 ug/mL antibody overnight, followed by incubation with biotinylated rabbit anti-mouse IgG conjugate. The immunocomplex was then visualised with DAB. Neurons, in the septal nuclei of basal forebrain, known to be cholinergic neurons, show strong staining for Ngfr.
Immunofluorescence
Immunofluorescent detection in cultured rat dorsal root ganglion (DRG) using Ngfr monoclonal antibody, clone MC192 (Cat # MAB5595). Merged pictures of cultured rat DRG triple-stained for Ngfr (red colour), beta-Tubulin (green colour) and nuclei using DAPI (violet colour).
Immunofluorescence
Immunofluorescent detection in cultured rat dorsal root ganglion (DRG). (A) Detection of Ngfr using Ngfr monoclonal antibody, clone MC192 (Cat # MAB5595). (B) Detection of betaIII-Tubulin. (C) DAPI staining. (D) Merged A, B and C.
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Specification
Product Description
Mouse monoclonal antibody raised against Ngfr.
Immunogen
Rat Ngfr.
Host
Mouse
Reactivity
Rat
Specificity
Specificity has been confirmed by immunohistochemical staining of lesioned sciatic nerve and spinal cord, the results reflect the reported literatures.
Form
Lyophilized
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from cell culture supernatant (1% BSA)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 100 uL sterilzed water, store at -20°C or lower. For an additional stability glycerol (1:1) may be added. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining in rat forebrain using Ngfr monoclonal antibody, clone MC192 (Cat # MAB5595). Paraformaldehyde fixed rat brain sections were incubated with 1 ug/mL antibody overnight, followed by incubation with biotinylated rabbit anti-mouse IgG conjugate. The immunocomplex was then visualised with DAB. Neurons, in the septal nuclei of basal forebrain, known to be cholinergic neurons, show strong staining for Ngfr.Immunofluorescence
Immunofluorescent detection in cultured rat dorsal root ganglion (DRG) using Ngfr monoclonal antibody, clone MC192 (Cat # MAB5595). Merged pictures of cultured rat DRG triple-stained for Ngfr (red colour), beta-Tubulin (green colour) and nuclei using DAPI (violet colour).Immunofluorescence
Immunofluorescent detection in cultured rat dorsal root ganglion (DRG). (A) Detection of Ngfr using Ngfr monoclonal antibody, clone MC192 (Cat # MAB5595). (B) Detection of betaIII-Tubulin. (C) DAPI staining. (D) Merged A, B and C.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ngfr
Entrez GeneID
24596Protein Accession#
P07174Gene Name
Ngfr
Gene Alias
LNGFR, RNNGFRR, p75, p75NTR
Gene Description
nerve growth factor receptor (TNFR superfamily, member 16)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
low affinity nerve growth factor receptor|nerve growth factor receptor, fast
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Publication Reference
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Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression.
Du Z, Bondarenko O, Wang D, Rouabhia M, Zhang Z.
Journal of Cellular Physiology 2016 Jun; 231(6):1301.
Application：IHC, Rat, Nerve.
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p75 and TrkA signaling regulates sympathetic neuronal firing patterns via differential modulation of voltage-gated currents.
Luther JA, Birren SJ.
Journal of Neuroscience 2009 Apr; 29(17):5411.
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Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation.
Lagares A, Li HY, Zhou XF, Avendano C.
Journal of Neuroscience 2007 Jul; 27(30):7939.
Application：IF, IHC, Rat, Rat trigeminal ganglion.
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Neural crest stem cells persist in the adult gut but undergo changes in self-renewal, neuronal subtype potential, and factor responsiveness.
Kruger GM, Mosher JT, Bixby S, Joseph N, Iwashita T, Morrison SJ.
Neuron 2002 Aug; 35(4):657.
Application：Flow Cyt, Rat, Neural crest stem cells.
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Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression.
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