FBLN5 monoclonal antibody, clone 3F10A5, 3F8A12
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FBLN5.
Immunogen
Recombinant protein corresponding to FBLN5.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:2000)
Immunohistochemistry (Frozen sections) (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using FBLN5 monoclonal antibody, clone 3F10A5, 3F8A12 (Cat # MAB5581) against truncated recombinant FBLN5 (Lane 1) and HeLa cell lysate (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma(A) and breast carcinoma (B) tissue, showing cytoplasmical localization, using FBLN5 monoclonal antibody, clone 3F10A5, 3F8A12 (Cat # MAB5581) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Fibulin-5 deposition in human skin: decrease with ageing and ultraviolet B exposure and increase in solar elastosis.
Kadoya K, Sasaki T, Kostka G, Timpl R, Matsuzaki K, Kumagai N, Sakai LY, Nishiyama T, Amano S.
The British Journal of Dermatology 2005 Sep; 153(3):607.
Application:IHC-P, Human, Human skin.
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Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection.
Wang X, LeMaire SA, Chen L, Carter SA, Shen YH, Gan Y, Bartsch H, Wilks JA, Utama B, Ou H, Thompson RW, Coselli JS, Wang XL.
Surgery. 2005 Aug; 138(2):352.
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Fibulin-5 promotes wound healing in vivo.
Lee MJ, Roy NK, Mogford JE, Schiemann WP, Mustoe TA.
Journal of the American College of Surgeons 2004 Sep; 199(3):403.
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Fibulin-5 deposition in human skin: decrease with ageing and ultraviolet B exposure and increase in solar elastosis.
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