MPO monoclonal antibody, clone 2C7
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Specification
Product Description
Mouse monoclonal antibody raised against MPO.
Immunogen
Human MPO.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. MON 8074 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. This antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. This antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Frozen sections) (1:1000-1: 5000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Flow Cytometry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of MPO in human bone marrow (formalin-fixed paraffin-embedded) using MPO monoclonal antibody, clone 2C7 (Cat. # MAB5342).Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — MPO
Entrez GeneID
4353Gene Name
MPO
Gene Alias
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Gene Description
myeloperoxidase
Gene Ontology
HyperlinkGene Summary
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Neutrophil specific granule and NETosis defects in gray platelet syndrome.
Cathelijn E M Aarts, Kate Downes, Arie J Hoogendijk, Evelien G G Sprenkeler, Roel P Gazendam, Rémi Favier, Marie Favier, Anton T J Tool, John L van Hamme, Myrto A Kostadima, Kate Waller, Barbara Zieger, Maaike G J M van Bergen, Saskia M C Langemeijer, Bert A van der Reijden, Hans Janssen, Timo K van den Berg, Robin van Bruggen, Alexander B Meijer, Willem H Ouwehand, Taco W Kuijpers.
Blood Advances 2021 Jan; 5(2):549.
Application:IEM, Human, Human neutrophils.
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Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
Gazendam RP, van Hamme JL, Tool AT, Hoogenboezem M, van den Berg JM, Prins JM, Vitkov L, van de Veerdonk FL, van den Berg TK, Roos D, Kuijpers TW.
The Journal of Immunology 2016 Feb; 196(3):1272.
Application:IF, Human, Neutrophils.
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Neutrophil specific granule and NETosis defects in gray platelet syndrome.
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