FKBP4 monoclonal antibody, clone AT4D3
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant FKBP4.
Immunogen
Recombinant protein corresponding to full length human FKBP4.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: K562 cell lysate, Lane 3: Jurkat cell lysate, Lane 4.: MCF7 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of seminoma (germ cell tumor) tissue were incubated with MAB3587 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunofluorescence
Immunofluorescence staining of HeLa cells line, stained with DAPI (Blue) for nucleus staining and FKBP4 monoclonal antibody, clone AT4D3 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cell line, staining at 2-5 ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — FKBP4
Entrez GeneID
2288Protein Accession#
NP_002005Gene Name
FKBP4
Gene Alias
FKBP52, FKBP59, HBI, Hsp56, PPIase, p52
Gene Description
FK506 binding protein 4, 59kDa
Omim ID
600611Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq
Other Designations
52 kD FK506 binding protein|FK506 binding protein 4 (59kD)|FK506 binding protein 52|FK506-binding protein 4 (59kD)|HSP binding immunophilin|T-cell FK506-binding protein, 59kD|p59 protein|peptidylprolyl cis-trans isomerase|rotamase
-
Interactome
-
Disease
-
Publication Reference
-
Pharmacologic unmasking of epigenetically silenced genes in breast cancer.
Ostrow KL, Park HL, Hoque MO, Kim MS, Liu J, Argani P, Westra W, Van Criekinge W, Sidransky D.
Clinical Cancer Research 2009 Feb; 15(4):1184.
-
The switch I region of Rheb is critical for its interaction with FKBP38.
Ma D, Bai X, Guo S, Jiang Y.
The Journal of Biological Chemistry 2008 Sep; 283(38):25963.
-
Pharmacologic unmasking of epigenetically silenced genes in breast cancer.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com