Cd40lg monoclonal antibody, clone MR1
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More Files
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Flow Cytometry
Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or plate-bound Cd3e monoclonal antibody, clone 145-2C11 for 7 hours at 37°C. The cells were then harvested, double-stained with Cd40lg monoclonal antibody, clone MR1 (Cat # MAB3231)-FITC and anti-CD4+8-R-PE, and analyzed by flow cytometry.
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Specification
Product Description
Hamster monoclonal antibody raised against Cd40lg.
Immunogen
Native Cd40lg from mouse Th1 clone D1.6.
Host
Hamster
Reactivity
Mouse
Specificity
CD154 (Mouse CD40 ligand/gp39), Mr 39-KDa.
Form
Liquid
Isotype
IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunoprecipitation
Flow Cytometry
Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or plate-bound Cd3e monoclonal antibody, clone 145-2C11 for 7 hours at 37°C. The cells were then harvested, double-stained with Cd40lg monoclonal antibody, clone MR1 (Cat # MAB3231)-FITC and anti-CD4+8-R-PE, and analyzed by flow cytometry. -
Gene Info — Cd40lg
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Publication Reference
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An essential role for gp39, the ligand for CD40, in thymic selection.
Foy TM, Page DM, Waldschmidt TJ, Schoneveld A, Laman JD, Masters SR, Tygrett L, Ledbetter JA, Aruffo A, Claassen E, Xu JC, Flavell RA, Oehen S, Hedrick SM, Noelle RJ.
The Journal of Experimental Medicine 1995 Nov; 182(5):1377.
Application：Flow Cyt, Func, Mouse, Mouse T cells, Mouse thymocytes.
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In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity. II. Prolonged suppression of the humoral immune response by an antibody to the ligand for CD40, gp39.
Foy TM, Shepherd DM, Durie FH, Aruffo A, Ledbetter JA, Noelle RJ.
The Journal of Experimental Medicine 1993 Nov; 178(5):1567.
Application：ELISA, Func, Mouse, Mouse serum.
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The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.
Aruffo A, Farrington M, Hollenbaugh D, Li X, Milatovich A, Nonoyama S, Bajorath J, Grosmaire LS, Stenkamp R, Neubauer M, et al..
Cell 1993 Jan; 72(2):291.
Application：Flow Cyt, IP, Human, Monkey, COS cells, Human T cells.
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An essential role for gp39, the ligand for CD40, in thymic selection.
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