His tag monoclonal antibody, clone 33D10.D2.G8 (HRP)
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More Files
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Western Blot
Western blot analysis with His tag monoclonal antibody, clone 33D10.D2.G8 (HRP) (Cat # MAB23676).
Lane 1: 100 ng purified histidine-tagged recombinant protein
Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct
Lane 3: 100 ng purified GST-tagged recombinant protein
Lane 4: 100 ng purified FLAG-tagged recombinant protein
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIS-tagged conjugated BSA at the N or C terminus of the His tag with His tag monoclonal antibody, clone 33D10.D2.G8 (HRP) (Cat # MAB23676).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with His tag monoclonal antibody, clone 33D10.D2.G8 (HRP) (Cat # MAB23676).
Antigen: HIS-tagged purified protein and E. coli cell lysates expressing His-tagged construct, GST- and RON-tagged purified proteins.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptides of His tag.
The antibody is conjugated with horseradish peroxidase (HRP).Immunogen
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Sequence
HHHHHH
Host
Mouse
Specificity
The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Form
Lyophilized
Conjugation
HRP
Purification
Protein A affinity purification
Concentration
1.0 mg/mL by UV abso
Isotype
IgG1
Recommend Usage
ELISA (1:10,000)
Immunohistochemistry (1:500 - 1:2,500)
Western Blot (1:1,000 - 1:5,000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (with 10 mg/mL BSA [immunoglobulin and protease free) and 0.01% (w/v) gentamicin sulfate).
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. -
Applications
Western Blot
Western blot analysis with His tag monoclonal antibody, clone 33D10.D2.G8 (HRP) (Cat # MAB23676).
Lane 1: 100 ng purified histidine-tagged recombinant protein
Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct
Lane 3: 100 ng purified GST-tagged recombinant protein
Lane 4: 100 ng purified FLAG-tagged recombinant proteinImmunohistochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIS-tagged conjugated BSA at the N or C terminus of the His tag with His tag monoclonal antibody, clone 33D10.D2.G8 (HRP) (Cat # MAB23676).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with His tag monoclonal antibody, clone 33D10.D2.G8 (HRP) (Cat # MAB23676).
Antigen: HIS-tagged purified protein and E. coli cell lysates expressing His-tagged construct, GST- and RON-tagged purified proteins.
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