NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (FITC)
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More Files
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Flow Cytometry
Flow cytometric analysis of CHO cells with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (FITC) (Cat # ).
(a) non-transfected CHO cells
(b) Strep II fusion protein-transfected CHO cells
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of NWSHPQFEK Tag (Strep II).
Antibody Species
Mouse
Immunogen
A synthetic peptide (conjugated with KLH) of NWSHPQFEK.
Specificity
This antibody recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
Form
Lyophilized
Conjugation
FITC
Purification
Protein A affinity purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1-3 µg for 1 x 106 cells)
Immunocytochemistry (1-3 ug/mL)
Immunofluorescence (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4 (containing 10 mg/mL BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C or below.
After reconstitution with deionized water, store at 4°C for 2-3 weeks, or at -20°C or below for long term storage.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Flow Cytometry
Flow cytometric analysis of CHO cells with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (FITC) (Cat # ).
(a) non-transfected CHO cells
(b) Strep II fusion protein-transfected CHO cells
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