NWSHPQFEK Tag monoclonal antibody, clone 5A9F9
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of N-terminal Strep II tagged fusion protein with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).
Immunofluorescence
Immunofluorescent staining of HEK293 cells with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).
Immunoprecipitation
Immunoprecipitation analysis of HEK293 cell lysates with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with RAB
Lane 3: Immunoprecipitates of non-transfected HEK293 cell lysates with RAB
Flow Cytometry
Flow cytometric analysis of CHO cells with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).
The affinity of for Strep II was analyzed in a flow cytometric analysis of non-transfected or Stresp II gene-transfected CHO cells (black and blue, respectively).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of NWSHPQFEK Tag (Strep II).
Antibody Species
Mouse
Immunogen
A synthetic peptide (conjugated with KLH) of NWSHPQFEK.
Specificity
This antibody recognizes NWSHPQFEK tags localized at the N-terminal and C-terminal region of Strep II tagged fusion proteins.
Form
Lyophilized
Purification
Protein A affinity purification
Isotype
IgG1, kappa
Recommend Usage
ELISA (0.001-0.002 ug/mL)
Flow Cytometry (1 ug/mL)
Immunocytochemistry (1-3 ug/mL)
Immunofluorescence (1-3 ug/mL)
Immunoprecipitation (1-5 ug/mL)
Western Blot (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4 (containing 0.02% sodium azide).
Storage Instruction
Store at -20°C for up to 1 year.
After reconstitution with deionized water, store at 4°C for 2-3 weeks, or at -20°C or below for long term storage.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of N-terminal Strep II tagged fusion protein with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of HEK293 cells with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).Immunoprecipitation
Immunoprecipitation analysis of HEK293 cell lysates with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with RAB
Lane 3: Immunoprecipitates of non-transfected HEK293 cell lysates with RABEnzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of CHO cells with NWSHPQFEK Tag monoclonal antibody, clone 5A9F9 (Cat # MAB23665).
The affinity of for Strep II was analyzed in a flow cytometric analysis of non-transfected or Stresp II gene-transfected CHO cells (black and blue, respectively).
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