SpCas9 Monoclonal Antibody, clone 14B6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with SpCas9 Monoclonal Antibody, clone 14B6.
Lane 1: 50 ug cell lysate of HEK293 transfected with PX458
Lane 2: 50 ug untransfected HEK293 cell lysate
Lane 3: 40 ug SpCas9 recombinant protein
Primary Antibody:
SpCas9 Monoclonal Antibody, clone 14B6 1 ug/mL
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 ug/mL
-
Specification
Product Description
Mouse monoclonal antibody raised against Streptococcus pyogenes Cas9.
Immunogen
Recombinant protein corresponding to Staphylococcus pyogenes Cas9.
Host
Mouse
Reactivity
Streptococcus pyogenes
Specificity
This antibody reacts to spCas9.
Form
Lyophilized
Purification
Protein A affinity purification
Isotype
IgG1 Kappa
Recommend Usage
ELISA (0.05-1 ug/mL)
Immunofluorescence (1-2 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (0.02% sodium azide).
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.Storage Instruction
Store at -20°C.
Upon reconstitution, it can be stored for up to 12 months at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with SpCas9 Monoclonal Antibody, clone 14B6.
Lane 1: 50 ug cell lysate of HEK293 transfected with PX458
Lane 2: 50 ug untransfected HEK293 cell lysate
Lane 3: 40 ug SpCas9 recombinant protein
Primary Antibody:
SpCas9 Monoclonal Antibody, clone 14B6 1 ug/mL
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 ug/mLEnzyme-linked Immunoabsorbent Assay
ELISA binding of SpCas9 Monoclonal Antibody, clone 14B6 with recombinant Streptococcus pyogenes CRISPR/Cas9 protein. Coating antigen: SpCas9, 1 ug/mL. SpCas9 Monoclonal Antibody, clone 14B6 dilution start from 3000 ng/mL, EC50= 24.51 ng/mL. -
Gene Info — SPy_1046
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com