GAPDH monoclonal antibody, clone 1D4
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More Files
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Western Blot (Tissue lysate)
Sciatic nerves of mouse wild type (1), heterozygous (2) and homozygous (3) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for western blots.
Blots were probed with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) to glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
Antibody was used at dilution of 1 : 1,000.
Signal was revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well.
Note the sharp clear band at 38 KDa, the expected molecular weight for GAPDH.
Immunofluorescence
Human neuroblastma line Sh-SY5Y stained with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) (green) and an anti-NF-H (red), counterstained with a fluorescent DNA probe (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against GAPDH.
Immunogen
Pig GAPDH.
Host
Mouse
Theoretical MW (kDa)
37
Reactivity
Birds, Bovine, Human, Mouse, Pig, Rat
Specificity
On Western Blot reveals a prominent ~37 KDa band.
Form
Liquid
Isotype
IgM
Quality Control Testing
Antibody Reactive Against GAPDH.
Recommend Usage
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (10 mM sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Sciatic nerves of mouse wild type (1), heterozygous (2) and homozygous (3) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for western blots.
Blots were probed with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) to glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
Antibody was used at dilution of 1 : 1,000.
Signal was revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well.
Note the sharp clear band at 38 KDa, the expected molecular weight for GAPDH.Immunocytochemistry
Immunofluorescence
Human neuroblastma line Sh-SY5Y stained with GAPDH monoclonal antibody, clone 1D4 (Cat # MAB2319) (green) and an anti-NF-H (red), counterstained with a fluorescent DNA probe (blue). -
Gene Info — GAPDH
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Publication Reference
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2-amino-N-{4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide (OSU-03012), a celecoxib derivative, directly targets p21-activated kinase.
Porchia LM, Guerra M, Wang YC, Zhang Y, Espinosa AV, Shinohara M, Kulp SK, Kirschner LS, Saji M, Chen CS, Ringel MD.
Molecular Pharmacology 2007 Aug; 72(5):1124.
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Apoptotic surge of potassium currents is mediated by p38 phosphorylation of Kv2.1.
Redman PT, He K, Hartnett KA, Jefferson BS, Hu L, Rosenberg PA, Levitan ES, Aizenman E.
PNAS 2007 Feb; 104(9):3568.
Application：WB-Ce, Rat, Rat cortical neurons.
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NAD(P)H oxidases regulate HIF-2alpha protein expression.
Block K, Gorin Y, Hoover P, Williams P, Chelmicki T, Clark RA, Yoneda T, Abboud HE.
The Journal of Biological Chemistry 2007 Mar; 282(11):8019.
Application：WB-Tr, Human, HK2, RCC 786-O cells.
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Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion.
Fatma S, Yakubov R, Anwar K, Hussain MM.
Journal of Lipid Research 2006 Nov; 47(11):2422.
Application：WB, Human, Caco-2 cells.
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Mitogen-activated protein kinases control cardiac KChIP2 gene expression.
Jia Y, Takimoto K.
Circulation Research 2006 Feb; 98(3):386.
Application：WB-Ti, Rat, Rat hearts.
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2-amino-N-{4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide (OSU-03012), a celecoxib derivative, directly targets p21-activated kinase.
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