DYKDDDDK tag monoclonal antibody, clone 29E4.G7 (HRP)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Mouse antibody for the detection of DYKDDDDK tag monoclonal antibody, clone 29E4.G7 (HRP) (Cat # MAB23148). Lane 1: FLAG Positive Control Lysates. Load: 10 ug per lane. Primary antibody: DYKDDDDK tag monoclonal antibody, clone 29E4.G7 (HRP) (Cat # MAB23148) at 1:1000 for overnight at 4°C. Secondary antibody: none. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: ~60 kDa, ~60 kDa.
-
Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptides of FLAG epitope tag. The antibody is conjugated with Horseradish Peroxidase (HRP).
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to FLAG epitope tag.
Sequence
DYKDDDDK
Host
Mouse
Specificity
Carboxy and amino terminal linked FLAG tagged recombinant proteins. This antibody is directed against the FLAG epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Form
Lyophilized
Conjugation
HRP
Preparation Method
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Isotype
IgG2a, kappa
Recommend Usage
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. -
Applications
Western Blot
Western blot analysis of Mouse antibody for the detection of DYKDDDDK tag monoclonal antibody, clone 29E4.G7 (HRP) (Cat # MAB23148). Lane 1: FLAG Positive Control Lysates. Load: 10 ug per lane. Primary antibody: DYKDDDDK tag monoclonal antibody, clone 29E4.G7 (HRP) (Cat # MAB23148) at 1:1000 for overnight at 4°C. Secondary antibody: none. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: ~60 kDa, ~60 kDa.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com