Drd5 monoclonal antibody, clone SG4-D1b
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More Files
- More Functions
Western Blot (Transfected lysate)
Detection of Drd5 of Sf9 cells transfected with rat Drd5 using Drd5 monoclonal antibody, clone SG4-D1b (Cat # MAB2313).
Lane 1 : Sf9-Drd1a lysate.
Lane 2 : Sf9-Drd5 lyate.
Please note that these results are similiar to those shown in J. Neuroimmunol. 101 : 170-187 publication.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Drd5.
Immunogen
Recombinant protein corresponding to the C-terminus last 118 amino acids of rat Drd5.
Host
Mouse
Theoretical MW (kDa)
52-64
Reactivity
Rat
Specificity
This antibody reacts with Dopamine Receptor D1B. This antibody is useful for Western Blot analysis, where a band is seen at 52-64 KDa in sf9 cell lysate.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Detection of Drd5 of Sf9 cells transfected with rat Drd5 using Drd5 monoclonal antibody, clone SG4-D1b (Cat # MAB2313).
Lane 1 : Sf9-Drd1a lysate.
Lane 2 : Sf9-Drd5 lyate.
Please note that these results are similiar to those shown in J. Neuroimmunol. 101 : 170-187 publication.Immunocytochemistry
Immunofluorescence
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Gene Info — Drd5
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Publication Reference
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Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes.
Luedtke RR, Griffin SA, Conroy SS, Jin X, Pinto A, Sesack SR.
Journal of Neuroimmunology 1999 Nov; 101(2):170.
Application：IHC, WB-Tr, Insect, Rat, Sf9 cells, Brain.
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Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes.
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