SIAH2 monoclonal antibody, clone 24E6H3
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More Files
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Immunohistochemistry
Immunohistochemical staining of SIAH2 on human colorectal adenocarcinoma with SIAH2 monoclonal antibody, clone 24E6H3 (Cat # MAB2298). Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
Immunofluorescence
Immunofluorescence staining of SIAH2 on NIH : OVCAR-3 with SIAH2 monoclonal antibody, clone 24E6H3 (Cat # MAB2298).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of SIAH2.
Immunogen
A synthetic peptide corresponding to amino acids 1-100 of drosophila SIAH2.
Host
Mouse
Reactivity
Fruit fly, Human, Pig
Specificity
This antibody is specific to human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This antibody does not detect Drosophila SINAH or normal, nondividing cells.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunocytochemistry (1:5-1:10)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:40-1:50)
Immunohistochemistry (Frozen sections) (1:40-1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunohistochemistry
Immunohistochemical staining of SIAH2 on human colorectal adenocarcinoma with SIAH2 monoclonal antibody, clone 24E6H3 (Cat # MAB2298). Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of SIAH2 on NIH : OVCAR-3 with SIAH2 monoclonal antibody, clone 24E6H3 (Cat # MAB2298).Flow Cytometry
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Publication Reference
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Effect of disrupting seven-in-absentia homolog 2 function on lung cancer cell growth.
Ahmed AU, Schmidt RL, Park CH, Reed NR, Hesse SE, Thomas CF, Molina JR, Deschamps C, Yang P, Aubry MC, Tang AH.
Journal of the National Cancer Institute 2008 Nov; 100(22):1606.
Application：IF, IHC-P, Human, A-549 cells, Human lung cancer.
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Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue.
Schmidt RL, Park CH, Ahmed AU, Gundelach JH, Reed NR, Cheng S, Knudsen BE, Tang AH.
Cancer Research 2007 Dec; 67(24):11798.
Application：IHC-P, Human, Panc-1, MiaPaCa tumors from Mouse.
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Effect of disrupting seven-in-absentia homolog 2 function on lung cancer cell growth.
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