CD274 monoclonal antibody, clone 15G3
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More Files
- More Functions
Western Blot (Recombinant protein)
Western Blot analysis of human cell expressed recombinant CD274 (40 ng). The blot was stained with HRP substrate 4-Chloro-1-Naphthol.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CD274.
Immunogen
Recombinant protein corresponding to amino acids 19-238 of human CD274 with poly-histidine tag at C-terminus.
Host
Mouse
Reactivity
Human
Specificity
Reactive with human CD274.
Form
Lyophilized
Purification
Protein G affinity purification
Isotype
IgG1, kappa
Recommend Usage
Capture Antibody
Sandwich ELISA (This antibody when coated on ELISA plates at 400 ng/well, and used in combination with CD274 monoclonal antibody, clone 12B4 (Cat # MAB22310) can detect human cell expressed CD274.)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.01 M PBS, pH 7.0.
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with double distilled water, store at 2-8°C for 2-4 weeks. For long term storage, aliquoted and stored at -20°C or lower temperature.
Aliquot to avoid repeated freezing and thawing.
Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL. -
Applications
Western Blot (Recombinant protein)
Western Blot analysis of human cell expressed recombinant CD274 (40 ng). The blot was stained with HRP substrate 4-Chloro-1-Naphthol.Sandwich ELISA
Capture Antibody
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Gene Info — CD274
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Interactome
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Pathway
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Disease
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