Hsc70 monoclonal antibody, clone 1B5
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Specification
Product Description
Rat monoclonal antibody raised against native Hsc70.
Immunogen
Native purified Hsc70 from Chinese hamster cells.
Host
Rat
Theoretical MW (kDa)
73
Reactivity
Birds, Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rat, Sheep
Specificity
Hsc70 purified from sodium arsenite treated heat-resistant variants. This antibody detects a 73 KDa protein on HeLa Heat Shocked Cell Lysate SDS-PAGE immunoblots, corresponding to the molecular weight of Hsc70.
Form
Liquid
Isotype
IgG2a
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Western Blot (0.1 ug/mL)
Immunoprecipitation (5 ug/mL)
Immunohistochemistry (5 ug/mL)
Immunocytochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 1 mM PMSF)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
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Publication Reference
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Characterization of mRNA profiles of the exosome-like vesicles in porcine follicular fluid.
Matsuno Y, Kanke T, Maruyama N, Fujii W, Naito K, Sugiura K.
PLoS One 2019 Jun; 14(6):e0217760.
Application：WB, Pig, Exosome-like vesicles in the exosomal fraction isolated from porcine follicular fluids.
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Effects of exosome-like vesicles on cumulus expansion in pigs in vitro.
Matsuno Y, Onuma A, Fujioka YA, Yasuhara K, Fujii W, Naito K, Sugiura K.
The Journal of Reproduction and Development 2017 Feb; 63(1):51.
Application：WB, Pig, Exosomal fraction and mural granulosa cells from Porcine ovaries.
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Characterization of mRNA profiles of the exosome-like vesicles in porcine follicular fluid.
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