VAMP3 monoclonal antibody, clone AT4G9
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: cellubrevin transfected 293T cell lysate, Lane 3: mouse brain tissue lysate.
Flow Cytometry
Flow cytometric analysis of HeLa cell line with VAMP3 monoclonal antibody, clone AT4G9 (Cat # MAB2061) staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant VAMP3.
Immunogen
Recombinant protein corresponding to amino acids 1-77 of human VAMP3.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: cellubrevin transfected 293T cell lysate, Lane 3: mouse brain tissue lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cell line with VAMP3 monoclonal antibody, clone AT4G9 (Cat # MAB2061) staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — VAMP3
Entrez GeneID
9341Protein Accession#
NP_004772Gene Name
VAMP3
Gene Alias
CEB
Gene Description
vesicle-associated membrane protein 3 (cellubrevin)
Omim ID
603657Gene Ontology
HyperlinkGene Summary
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq
Other Designations
OTTHUMP00000001361|cellubrevin|synaptobrevin-3|vesicle-associated membrane protein 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion.
Proux-Gillardeaux V, Gavard J, Irinopoulou T, Mège RM, Galli T.
PNAS 2005 May; 102(18):6362.
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VAMP2, but not VAMP3/cellubrevin, mediates insulin-dependent incorporation of GLUT4 into the plasma membrane of L6 myoblasts.
Randhawa VK, Bilan PJ, Khayat ZA, Daneman N, Liu Z, Ramlal T, Volchuk A, Peng XR, Coppola T, Regazzi R, Trimble WS, Klip A.
Molecular Biology of the Cell 2000 Jul; 11(7):2403.
Application：IF, Rat, L6 myoblasts.
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Syntaxin 4, VAMP2, and/or VAMP3/cellubrevin are functional target membrane and vesicle SNAP receptors for insulin-stimulated GLUT4 translocation in adipocytes.
Olson AL, Knight JB, Pessin JE.
Molecular and Cellular Biology 1997 May; 17(5):2425.
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Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion.
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