HTB1 (phospho T129) monoclonal antibody, clone DEI-8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate using HTB1 (phospho T129) monoclonal antibody, clone DEI-8.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of yeast HTB1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding T129 of yeast HTB1.
Host
Rabbit
Reactivity
Yeast
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate using HTB1 (phospho T129) monoclonal antibody, clone DEI-8. -
Gene Info — HTB1
Entrez GeneID
851810Protein Accession#
P02293Gene Name
HTB1
Gene Alias
SPT12
Gene Description
Htb1p
Gene Ontology
HyperlinkGene Summary
meiotic DSB formation and H3 methylation
Other Designations
One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
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