PPIB monoclonal antibody, clone k2E2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: K562 cell lysate, Lane 3: 293T cell lysate, Lane 4: A549 cell lysate, Lane 5: MCF7 cell lysate, Lane 6: SK-OV-3 cell lysate, Lane 7: LnCap cell lysate.
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with PPIB monoclonal antibody, clone k2E2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PPIB.
Immunogen
Recombinant protein corresponding to amino acids 26-216 of human PPIB.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: K562 cell lysate, Lane 3: 293T cell lysate, Lane 4: A549 cell lysate, Lane 5: MCF7 cell lysate, Lane 6: SK-OV-3 cell lysate, Lane 7: LnCap cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with PPIB monoclonal antibody, clone k2E2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — PPIB
Entrez GeneID
5479Protein Accession#
P23284Gene Name
PPIB
Gene Alias
CYP-S1, CYPB, MGC14109, MGC2224, SCYLP
Gene Description
peptidylprolyl isomerase B (cyclophilin B)
Omim ID
123841Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. [provided by RefSeq
Other Designations
PPIase|S-cyclophilin|cyclophilin B|cyclophilin-like protein|peptidyl-prolyl cis-trans isomerase B|peptidylprolyl isomerase B|rotamase
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Interactome
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Disease
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Publication Reference
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A systems wide mass spectrometric based linear motif screen to identify dominant in-vivo interacting proteins for the ubiquitin ligase MDM2.
Nicholson J, Scherl A, Way L, Blackburn EA, Walkinshaw MD, Ball KL, Hupp TR.
Cellular Signalling 2014 Jun; 26(6):1243.
Application：ELISA, WB, Human, MCF-7 cells.
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Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase.
Watashi K, Ishii N, Hijikata M, Inoue D, Murata T, Miyanari Y, Shimotohno K.
Molecular Cell 2005 Jul; 19(1):111.
Application：IF, IP, WB-Tr, Human, Huh7, MH-14 cells.
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CD147 is a signaling receptor for cyclophilin B.
Yurchenko V, O'Connor M, Dai WW, Guo H, Toole B, Sherry B, Bukrinsky M.
Biochemical and Biophysical Research Communications 2001 Nov; 288(4):786.
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Human cyclophilin B: a second cyclophilin gene encodes a peptidyl-prolyl isomerase with a signal sequence.
Price ER, Zydowsky LD, Jin MJ, Baker CH, McKeon FD, Walsh CT.
PNAS 1991 Mar; 88(5):1903.
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A systems wide mass spectrometric based linear motif screen to identify dominant in-vivo interacting proteins for the ubiquitin ligase MDM2.
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