LEFTY2 monoclonal antibody, clone 7C5G1H6H10
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant LEFTY2.
Immunogen
Recombinant His fusion protein corresponding to human LEFTY2.
Host
Mouse
Reactivity
Human
Specificity
No reactivity occurs against 6X HIS tag.
Form
Liquid
Purification
This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY.
Isotype
IgG2a
Recommend Usage
ELISA (1:5000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store vial at -20°C prior to opening. For long term storage, aliquot contents and freeze at -20°C or below.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
LEFTY2 monoclonal antibody, clone 7C5G1H6H10 (Cat # MAB2032) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY2.
Detection occurs after 1.0 ug of protein is loaded in each lane.
The blot was incubated with a 1:2,000 dilution of LEFTY2 monoclonal antibody, clone 7C5G1H6H10 at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1 : 1,000.
Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag.
Lane 2 contains partially purified recombinant human LEFTY.
The antibody may be used to detect endogenous human LEFTY.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LEFTY2
Entrez GeneID
7044Gene Name
LEFTY2
Gene Alias
EBAF, LEFTA, LEFTYA, MGC46222, TGFB4
Gene Description
left-right determination factor 2
Omim ID
601877Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq
Other Designations
OTTHUMP00000035570|endometrial bleeding associated factor|endometrial bleeding associated factor (left-right determination, factor A; transforming growth factor beta superfamily)|transforming growth factor, beta-4 (endometrial bleeding-associated factor;
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Interactome
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Pathway
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Disease
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Publication Reference
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Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor.
Ulloa L, Tabibzadeh S.
The Journal of Biological Chemistry 2001 Jun; 276(24):21397.
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Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1.
Tsukui T, Capdevila J, Tamura K, Ruiz-Lozano P, Rodriguez-Esteban C, Yonei-Tamura S, Magallon J, Chandraratna RA, Chien K, Blumberg B, Evans RM, Belmonte JC.
PNAS 1999 Sep; 96(20):11376.
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lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal.
Meno C, Shimono A, Saijoh Y, Yashiro K, Mochida K, Ohishi S, Noji S, Kondoh H, Hamada H.
Cell 1998 Aug; 94(3):287.
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Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor.
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