CDX2 monoclonal antibody, clone AFA-3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell lysate; (2) Colon cancer lysate with CDX2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human colon with CDX2 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CDX2.
Immunogen
A synthetic peptide corresponding to human CDX2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell lysate; (2) Colon cancer lysate with CDX2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human colon with CDX2 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — CDX2
Entrez GeneID
1045Protein Accession#
Q99626Gene Name
CDX2
Gene Alias
CDX-3, CDX3
Gene Description
caudal type homeobox 2
Omim ID
600297Gene Ontology
HyperlinkGene Summary
The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM
Other Designations
OTTHUMP00000018176|caudal type homeo box transcription factor 2|caudal type homeobox transcription factor 2
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Interactome
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Disease
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