CENPE monoclonal antibody, clone 1H12
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More Files
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Immunofluorescence
Immunofluorescence microscopy of CENPE monoclonal antibody, clone 1H12 (Cat # MAB1924) was used to detect CENPE protein visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A) .
Interphase cells show no discrete staining (bottom left, panel B) .
HeLa cells were fixed in paraformaldehyde and stained using this primary antibody.
AlexaFluor 555 conjugated anti-Mouse antibody (red) was used for detection.
DNA was stained using bis-benzimide (DAPI) (green) .
Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant.
Immunogen
Recombinant protein corresponding to full length human CENPE.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:500-1:2000)
Immunoprecipitation (1:200)
Immunofluorescence (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunofluorescence
Immunofluorescence microscopy of CENPE monoclonal antibody, clone 1H12 (Cat # MAB1924) was used to detect CENPE protein visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A) .
Interphase cells show no discrete staining (bottom left, panel B) .
HeLa cells were fixed in paraformaldehyde and stained using this primary antibody.
AlexaFluor 555 conjugated anti-Mouse antibody (red) was used for detection.
DNA was stained using bis-benzimide (DAPI) (green) .
Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CENPE
Entrez GeneID
1062Protein Accession#
P49840;NP_063937Gene Name
CENPE
Gene Alias
KIF10
Gene Description
centromere protein E, 312kDa
Omim ID
117143Gene Ontology
HyperlinkGene Summary
Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq
Other Designations
Centromere autoantigen E (312kD)|centromere protein E|centromere protein E (312kD)|kinesin family member 10
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Interactome
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Disease
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Publication Reference
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Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.
Chan GK, Schaar BT, Yen TJ.
The Journal of Cell Biology 1998 Oct; 143(1):49.
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CENP-E is a putative kinetochore motor that accumulates just before mitosis.
Yen TJ, Li G, Schaar BT, Szilak I, Cleveland DW.
Nature 1992 Oct; 359(6395):536.
Application：IF, IP, WB-Tr, Human, HeLa cells.
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CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase.
Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, Cleveland DW.
The EMBO Journal 1991 May; 10(5):1245.
Application：IF, WB-Ce, Human, HeLa cells.
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Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.
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