JMJD1A monoclonal antibody, clone 3G10C2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) JMJD1A-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JMJD1A monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using JMJD1A mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human JMJD1A.
Immunogen
Recombinant protein corresponding to amino acids 311-445 of human JMJD1A from E. coli.
Host
Mouse
Theoretical MW (kDa)
147
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunofluorescence (1:50-250)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) JMJD1A-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JMJD1A monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using JMJD1A mouse monoclonal antibody (green) and negative control (red). -
Gene Info — JMJD1A
Entrez GeneID
55818Gene Name
JMJD1A
Gene Alias
DKFZp686A24246, DKFZp686P07111, JHDM2A, JHMD2A, JMJD1, KDM3A, KIAA0742, TSGA
Gene Description
jumonji domain containing 1A
Omim ID
611512Gene Ontology
HyperlinkGene Summary
This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000160707|jumonji C domain-containing histone demethylase 2A|testis-specific protein A
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Interactome
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Disease
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