UHRF1 monoclonal antibody, clone 2A8C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) HCT116 cell, (3) HL-60 cell, (4) HeLa cell, (5) HEK293 cell with UHRF1 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) UHRF1-hIgGFc transfected HEK293 cell lysate with UHRF1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with UHRF1 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of UHRF1 monoclonal antibody, clone 2A8C7.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human UHRF1.
Immunogen
Recombinant protein corresponding to amino acids 616-755 of human UHRF1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
148.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) HCT116 cell, (3) HL-60 cell, (4) HeLa cell, (5) HEK293 cell with UHRF1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) UHRF1-hIgGFc transfected HEK293 cell lysate with UHRF1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with UHRF1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of UHRF1 monoclonal antibody, clone 2A8C7. -
Gene Info — UHRF1
Entrez GeneID
29128Gene Name
UHRF1
Gene Alias
FLJ21925, ICBP90, MGC138707, Np95, RNF106, hNP95
Gene Description
ubiquitin-like with PHD and ring finger domains 1
Omim ID
607990Gene Ontology
HyperlinkGene Summary
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
E3 ubiquitin-protein ligase UHRF1|RING finger protein 106|inverted CCAAT box-binding protein of 90 kDa|nuclear zinc finger protein Np95|transcription factor ICBP90|ubiquitin-like, containing PHD and RING finger domains, 1
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Interactome
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Disease
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