HTR3B monoclonal antibody, clone 2H9H5
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human HTR3B.
Immunogen
Recombinant protein corresponding to amino acid 23-150 of human HTR3B from E. coli.
Host
Mouse
Theoretical MW (kDa)
50.3
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) SK-N-SH cell, (2) Hela cell with HTR3B monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) HTR3B-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with HTR3B monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HTR3B monoclonal antibody, clone 2H9H5.Flow Cytometry
Flow cytometric analysis of Hela cells with HTR3B monoclonal antibody (green) and negative control (red). -
Gene Info — HTR3B
Entrez GeneID
9177Gene Name
HTR3B
Gene Alias
5-HT3B
Gene Description
5-hydroxytryptamine (serotonin) receptor 3B
Omim ID
604654Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit B of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. [provided by RefSeq
Other Designations
5-hydroxytryptamine 3 receptor B subunit|serotonin-gated ion channel subunit
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Interactome
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Disease
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