TH monoclonal antibody, clone 1B8D2
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) SH-SY5Y, and (2) PC-12 cell lysate.
Western Blot
Western Blot analysis of human TH recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of cervical cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TH monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using TH mouse monoclonal antibody (green) and negative control (red).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using TH mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human TH.
Immunogen
Recombinant protein corresponding to amino acids 44-208 of of human TH from E. coli.
Host
Mouse
Theoretical MW (kDa)
58.6
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) SH-SY5Y, and (2) PC-12 cell lysate.Western Blot
Western Blot analysis of human TH recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of cervical cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TH monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using TH mouse monoclonal antibody (green) and negative control (red).Flow Cytometry
Flow cytometric analysis of MCF-7 cells using TH mouse monoclonal antibody (green) and negative control (red). -
Gene Info — TH
Entrez GeneID
7054Gene Name
TH
Gene Alias
TYH
Gene Description
tyrosine hydroxylase
Omim ID
191290Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000011225|OTTHUMP00000011226|tyrosine 3-monooxygenase
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Interactome
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Pathway
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Disease
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