DAB2 monoclonal antibody, clone 2H7C4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DAB2-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with DAB2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DAB2 monoclonal antibody, clone 2H7C4.
Flow Cytometry
Flow cytometric analysis of HeLa cells with DAB2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DAB2.
Immunogen
Recombinant protein corresponding to amino acid 652-749 of human DAB2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
82.4kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DAB2-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with DAB2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DAB2 monoclonal antibody, clone 2H7C4.Flow Cytometry
Flow cytometric analysis of HeLa cells with DAB2 monoclonal antibody (green) and negative control (red). -
Gene Info — DAB2
Entrez GeneID
1601Gene Name
DAB2
Gene Alias
DOC-2, DOC2, FLJ26626
Gene Description
disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
Omim ID
601236Gene Ontology
HyperlinkGene Summary
DAB2 mRNA is expressed in normal ovarian epithelial cells but is down-regulated or absent from ovarian carcinoma cell lines. The 770-amino acid predicted protein has an overall 83% identity with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain. The down-regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). [provided by RefSeq
Other Designations
disabled homolog 2|mitogen-responsive phosphoprotein
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Interactome
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Pathway
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Disease
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