ZFP91 monoclonal antibody, clone 8C3D5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell; Lane 2: A431 cell; Lane 3: HepG2 cell; Lane 4: HEK293 cell; Lane 5: A549 cell; Lane 6: PC-3 cell with ZFP91 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ZFP91-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with ZFP91 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ZFP91 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ZFP91 monoclonal antibody, clone 8C3D5.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ZFP91 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ZFP91.
Immunogen
Recombinant protein corresponding to amino acid 162-304 of human ZFP91 from E. coli.
Host
Mouse
Theoretical MW (kDa)
63.4kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:100- 1:500)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell; Lane 2: A431 cell; Lane 3: HepG2 cell; Lane 4: HEK293 cell; Lane 5: A549 cell; Lane 6: PC-3 cell with ZFP91 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ZFP91-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with ZFP91 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ZFP91 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ZFP91 monoclonal antibody, clone 8C3D5.Flow Cytometry
Flow cytometric analysis of HeLa cells with ZFP91 monoclonal antibody (green) and negative control (red). -
Gene Info — ZFP91
Entrez GeneID
80829Gene Name
ZFP91
Gene Alias
FKSG11, PZF, ZNF757
Gene Description
zinc finger protein 91 homolog (mouse)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the zinc finger family of proteins. The gene product contains C2H2 type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. A pseudogene has also been identified on chromosome 2. [provided by RefSeq
Other Designations
zinc finger protein 91|zinc finger protein homologous to Zfp91 in mouse
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Interactome
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Disease
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