ETF1 monoclonal antibody, clone 4F9H12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: T47D cell; Lane 3: MOLT4 cell; Lane 4: Raji cell with ETF1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ETF1-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ETF1 monoclonal antibody, clone 4F9H12.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ETF1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ETF1.
Immunogen
Recombinant protein corresponding to amino acid 288-437 of human ETF1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
49kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: T47D cell; Lane 3: MOLT4 cell; Lane 4: Raji cell with ETF1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ETF1-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ETF1 monoclonal antibody, clone 4F9H12.Flow Cytometry
Flow cytometric analysis of HeLa cells with ETF1 monoclonal antibody (green) and negative control (red). -
Gene Info — ETF1
Entrez GeneID
2107Gene Name
ETF1
Gene Alias
D5S1995, ERF, ERF1, MGC111066, RF1, SUP45L1, TB3-1
Gene Description
eukaryotic translation termination factor 1
Omim ID
600285Gene Ontology
HyperlinkGene Summary
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM
Other Designations
polypeptide chain release factor 1|sup45 (yeast omnipotent suppressor 45) homolog-like 1
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Interactome
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Disease
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