RAD23A monoclonal antibody, clone 3E3F4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD23A-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with RAD23A monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD23A monoclonal antibody, clone 3E3F4.
Flow Cytometry
Flow cytometric analysis of HeLa cells with RAD23A monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAD23A.
Immunogen
Recombinant protein corresponding to amino acid 1-363 of human RAD23A from E. coli.
Host
Mouse
Theoretical MW (kDa)
40kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD23A-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with RAD23A monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD23A monoclonal antibody, clone 3E3F4.Flow Cytometry
Flow cytometric analysis of HeLa cells with RAD23A monoclonal antibody (green) and negative control (red). -
Gene Info — RAD23A
Entrez GeneID
5886Gene Name
RAD23A
Gene Alias
HHR23A, MGC111083
Gene Description
RAD23 homolog A (S. cerevisiae)
Omim ID
600061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq
Other Designations
RAD23, yeast homolog, A|UV excision repair protein RAD23 homolog A
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Interactome
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Pathway
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Disease
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