SPHK2 monoclonal antibody, clone 9C5E1
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with SPHK2 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with SPHK2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SPHK2 monoclonal antibody, clone 9C5E1.
Flow Cytometry
Flow cytometric analysis of HeLa cells with SPHK2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human SPHK2.
Immunogen
A synthetic peptide corresponding to amino acid 36-52 of human SPHK2.
Host
Mouse
Theoretical MW (kDa)
69.2kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with SPHK2 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with SPHK2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SPHK2 monoclonal antibody, clone 9C5E1.Flow Cytometry
Flow cytometric analysis of HeLa cells with SPHK2 monoclonal antibody (green) and negative control (red). -
Gene Info — SPHK2
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Interactome
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Pathway
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