CD274 monoclonal antibody, clone RM320
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD274.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus of human CD274.
Reactivity
Human
Specificity
This antibody reacts to human PD-L1 (B7-H1).
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:200-500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) H1975 cell lysates, (2) DU145 cell lysates and (3) LNCaP cell lysates.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lung cancer.Immunohistochemistry
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using CD274 monoclonal antibody, clone RM320 (Cat# MAB16935) at a 1:250 dilution. -
Gene Info — CD274
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Interactome
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Pathway
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Disease
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Publication Reference
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The miR-3127-5p/p-STAT3 axis up-regulates PD-L1 inducing chemoresistance in non-small-cell lung cancer.
Tang D, Zhao D, Wu Y, Yao R, Zhou L, Lu L, Gao W, Sun Y.
Journal of Cellular and Molecular Medicine 2018 May; [Epub].
Application:WB, Human, A549, H1299 cells.
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The miR-3127-5p/p-STAT3 axis up-regulates PD-L1 inducing chemoresistance in non-small-cell lung cancer.
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