Syt7 monoclonal antibody, clone S275-14 (Biotin)
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More Files
- More Functions
Western Blot (Tissue lysate)
Western Blot analysis of rat brain membrane lysate (Lane 1: 1:100, Lane 2: 1:250, Lane 3: 1:500 and Lane 4: 1:1000) with Syt7 monoclonal antibody, clone S275-14 (Biotin) (Cat # MAB16826).
Immunocytochemistry
Immunocytochemical staining of SK-N-BE with Syt7 monoclonal antibody, clone S275-14 (Biotin) (Cat # MAB16826). (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Syt7 Antibody and (D) Composite.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant mouse Syt7.
Immunogen
Recombinant protein corresponding to amino acids 150-239 at cytoplasmic C2A domain of mouse Syt7.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Conjugation
Biotin
Purification
Protein G purification
Isotype
IgG2b
Recommend Usage
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of rat brain membrane lysate (Lane 1: 1:100, Lane 2: 1:250, Lane 3: 1:500 and Lane 4: 1:1000) with Syt7 monoclonal antibody, clone S275-14 (Biotin) (Cat # MAB16826).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunocytochemical staining of SK-N-BE with Syt7 monoclonal antibody, clone S275-14 (Biotin) (Cat # MAB16826). (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Syt7 Antibody and (D) Composite.Immunofluorescence
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Gene Info — Syt7
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Publication Reference
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Botulinum neurotoxin A activity is dependent upon the presence of specific gangliosides in neuroblastoma cells expressing synaptotagmin I.
Brian C Yowler, Richard D Kensinger, Cara-Lynne Schengrund.
The Journal of Biological Chemistry 2002 Sep; 277(36):32815.
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Botulinum neurotoxin A activity is dependent upon the presence of specific gangliosides in neuroblastoma cells expressing synaptotagmin I.
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