TFAP2A monoclonal antibody, clone 1A10C5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: TFAP2A-hIgGFc transfected HEK293 cell with TFAP2A monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with TFAP2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TFAP2A monoclonal antibody, clone 1A10C5.
Flow Cytometry
Flow cytometric analysis of Hela cells with TFAP2A monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human TFAP2A.
Immunogen
Recombinant protein corresponding to amino acid 1-100 of human TFAP2A from E. coli.
Host
Mouse
Theoretical MW (kDa)
48
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: TFAP2A-hIgGFc transfected HEK293 cell with TFAP2A monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with TFAP2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TFAP2A monoclonal antibody, clone 1A10C5.Flow Cytometry
Flow cytometric analysis of Hela cells with TFAP2A monoclonal antibody (green) and negative control (red). -
Gene Info — TFAP2A
Entrez GeneID
7020Gene Name
TFAP2A
Gene Alias
AP-2, AP-2alpha, AP2TF, BOFS, TFAP2
Gene Description
transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Omim ID
107580Gene Ontology
HyperlinkGene Summary
AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM
Other Designations
OTTHUMP00000016013|OTTHUMP00000016014|activating enhancer-binding protein 2 alpha|transcription factor AP-2 alpha|transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
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Interactome
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Disease
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