COX4I1 monoclonal antibody, clone CL3497
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: HEK 293, Lane 3: A-431, Lane 4: HepG2, Lane 5: NIH-3T3 and Lane 6: NBT-II cell lysates with COX4I1 monoclonal antibody, clone CL3497 (Cat # MAB15804).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with COX4I1 monoclonal antibody, clone CL3497 (Cat # MAB15804) shows strong cytoplasmic immunoreactivity in glandular epithelium cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human COX4I1.
Immunogen
Recombinant protein corresponding to human COX4I1.
Epitope
This antibody binds to an epitope located within the peptide sequence VVKSEDFSLP as determined by overlapping synthetic peptides.
Sequence
LATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKT
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: HEK 293, Lane 3: A-431, Lane 4: HepG2, Lane 5: NIH-3T3 and Lane 6: NBT-II cell lysates with COX4I1 monoclonal antibody, clone CL3497 (Cat # MAB15804).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with COX4I1 monoclonal antibody, clone CL3497 (Cat # MAB15804) shows strong cytoplasmic immunoreactivity in glandular epithelium cells. -
Gene Info — COX4I1
Entrez GeneID
1327Protein Accession#
P13073Gene Name
COX4I1
Gene Alias
COX4, COXIV, MGC72016
Gene Description
cytochrome c oxidase subunit IV isoform 1
Omim ID
123864Gene Ontology
HyperlinkGene Summary
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq
Other Designations
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Interactome
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