SETDB1 monoclonal antibody, clone 5H6A12
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Line 1: MCF-7, Line 2: T47D, Line 3: HEK293, Line 4: JURKAT, Line 5: NIH/3T3, Line 6: F9, Line 7: RAW246.7 and Line8: Cos7 cell lysate.
Immunofluorescence
Immunofluorescence staining of LOVO cells using SETDB1 monoclonal antibody, clone 5H6A12 (Green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human SETDB1.
Immunogen
Recombinant protein corresponding to human SETDB1.
Host
Mouse
Reactivity
Human, Monkey, Mouse
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Line 1: MCF-7, Line 2: T47D, Line 3: HEK293, Line 4: JURKAT, Line 5: NIH/3T3, Line 6: F9, Line 7: RAW246.7 and Line8: Cos7 cell lysate.Immunofluorescence
Immunofluorescence staining of LOVO cells using SETDB1 monoclonal antibody, clone 5H6A12 (Green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SETDB1
Entrez GeneID
9869Protein Accession#
Q15047Gene Name
SETDB1
Gene Alias
ESET, KG1T, KIAA0067, KMT1E
Gene Description
SET domain, bifurcated 1
Omim ID
604396Gene Ontology
HyperlinkGene Summary
This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described
Other Designations
ERG-associated protein with a SET domain, ESET
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Interactome
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Pathway
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Disease
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