TOP2A monoclonal antibody, clone TOP2A/1361
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TOP2A.
Immunogen
Recombinant protein corresponding to amino acids 1352-1493 of human TOP2A.
Host
Mouse
Theoretical MW (kDa)
170
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HepG2, Lane 2: HeLa and Lane 3: 3T3 cell lysates with TOP2A monoclonal antibody, clone TOP2A/1361 (Cat # MAB15012).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil (A) and human bladder carcinoma (B) with TOP2A monoclonal antibody, clone TOP2A/1361 (Cat # MAB15012).Immunofluorescence
Flow Cytometry
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Gene Info — TOP2A
Entrez GeneID
7153Protein Accession#
P11388Gene Name
TOP2A
Gene Alias
TOP2, TP2A
Gene Description
topoisomerase (DNA) II alpha 170kDa
Omim ID
126430Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq
Other Designations
DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha (170kD)
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Interactome
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Disease
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Publication Reference
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Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes.
Chung TD, Drake FH, Tan KB, Per SR, Crooke ST, Mirabelli CK.
PNAS 1989 Dec; 86(23):9431.
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Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes.
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