PODXL monoclonal antibody, clone 4F10
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PODXL.
Immunogen
Recombinant protein corresponding to intracellular, transmembrane, and part of the extracellular domain of human PODXL.
Host
Mouse
Theoretical MW (kDa)
165-170
Reactivity
Human
Form
Liquid
Isotype
IgM, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human angiosarcoma with PODXL monoclonal antibody, clone 4F10 (Cat # MAB14398).Immunofluorescence
Flow Cytometry
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Gene Info — PODXL
Entrez GeneID
5420Protein Accession#
O00592Gene Name
PODXL
Gene Alias
Gp200, MGC138240, PC, PCLP
Gene Description
podocalyxin-like
Omim ID
602632Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq
Other Designations
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Interactome
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Disease
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