DAG1 (phospho Y892) monoclonal antibody, clone M117
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 min (lane 2). Blots were probed with DAG1 (phospho Y892) monoclonal antibody, clone M117 (Cat # MAB1368).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of DAG1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y892 of human DAG1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence has high homology to the conserved tyrosine site in rat and mouse dystroglycan.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 min (lane 2). Blots were probed with DAG1 (phospho Y892) monoclonal antibody, clone M117 (Cat # MAB1368).Enzyme-linked Immunoabsorbent Assay
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Gene Info — DAG1
Entrez GeneID
1605Gene Name
DAG1
Gene Alias
156DAG, A3a, AGRNR, DAG
Gene Description
dystroglycan 1 (dystrophin-associated glycoprotein 1)
Omim ID
128239Gene Ontology
HyperlinkGene Summary
Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants
Other Designations
alpha-dystroglycan|beta-dystroglycan|dystroglycan 1|dystrophin-associated glycoprotein-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Localization of phospho-beta-dystroglycan (pY892) to an intracellular vesicular compartment in cultured cells and skeletal muscle fibers in vivo.
Sotgia F, Bonuccelli G, Bedford M, Brancaccio A, Mayer U, Wilson MT, Campos-Gonzalez R, Brooks JW, Sudol M, Lisanti MP.
Biochemistry 2003 Jun; 42(23):7110.
Application：IF, WB, Monkey, Mouse, COS-7 cells, Skeletal muscle fibers.
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Adhesion-dependent tyrosine phosphorylation of (beta)-dystroglycan regulates its interaction with utrophin.
James M, Nuttall A, Ilsley JL, Ottersbach K, Tinsley JM, Sudol M, Winder SJ.
Journal of Cell Science 2000 May; 113(Pt10):1717.
Application：WB-Ce, Human, HeLa cells.
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Localization of phospho-beta-dystroglycan (pY892) to an intracellular vesicular compartment in cultured cells and skeletal muscle fibers in vivo.
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