PXN monoclonal antibody, clone M107
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431 cells (20 mg/lane) serum starved overnight. Then untreated (lane 1) or treated (lane 2) with EGF (100 ng/ml) for 5 min. The blot was probed with PXN monoclonal antibody, clone M107 (Cat # MAB1366).
Immunofluorescence
Immunocytochemical labeling of PXN in rabbit spleen fibroblasts. The cells were labeled with PXN monoclonal antibody, clone M107 (Cat # MAB1366) (left) , then detected using appropriate secondary antibodies conjugated to Cy3.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant PXN.
Immunogen
Recombinant protein corresponding to full length human PXN.
Host
Mouse
Reactivity
Chicken, Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:100)
Immunoprecipitation (1-5 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cells (20 mg/lane) serum starved overnight. Then untreated (lane 1) or treated (lane 2) with EGF (100 ng/ml) for 5 min. The blot was probed with PXN monoclonal antibody, clone M107 (Cat # MAB1366).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling of PXN in rabbit spleen fibroblasts. The cells were labeled with PXN monoclonal antibody, clone M107 (Cat # MAB1366) (left) , then detected using appropriate secondary antibodies conjugated to Cy3.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PXN
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Interactome
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Pathway
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Disease
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Publication Reference
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A role for JNK-paxillin signaling in cell migration.
Huang C, Jacobson K, Schaller MD.
Cell Cycle 2004 Jan; 3(1):4.
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Ras-induced serine phosphorylation of the focal adhesion protein paxillin is mediated by the Raf-->MEK-->ERK pathway.
Woodrow MA, Woods D, Cherwinski HM, Stokoe D, McMahon M.
Experimental Cell Research 2003 Jul; 287(2):325.
Application：IF, IP, WB, Mouse, NIH/3T3 cells.
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JNK phosphorylates paxillin and regulates cell migration.
Huang C, Rajfur Z, Borchers C, Schaller MD, Jacobson K.
Nature 2003 Jul; 424(6945):219.
Application：WB-Tr, Human, HEK 293, MDA-MB-231 cells.
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A role for JNK-paxillin signaling in cell migration.
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