Phosphotyrosine monoclonal antibody, clone RM111
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of serum-starved A431 cells nontreated or treated with 100 ng/mL EGF using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) at 1:5000 dilution.
Immunocytochemistry
Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with EGF (B) using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).
Immunoprecipitation
Immunoprecipitation analysis of Lane 1: EGE-treated A431 cell whole lysates, Lane 2: EGE-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: EGE-treated A431 cell whole lysates using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) at 1:1000 dilution.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against phosphotyrosine.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against phosphotyrosine conjugated with BSA.
Sequence
N/A
Specificity
This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:200-1:500)
Immunohistochemistry (1:200-1:500)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of serum-starved A431 cells nontreated or treated with 100 ng/mL EGF using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) at 1:5000 dilution.Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with EGF (B) using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).Immunoprecipitation
Immunoprecipitation analysis of Lane 1: EGE-treated A431 cell whole lysates, Lane 2: EGE-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: EGE-treated A431 cell whole lysates using Phosphotyrosine monoclonal antibody, clone RM111 (Cat # MAB12826) at 1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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