Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103
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More Files
- More Functions
Western Blot
Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat # MAB12764) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (kappa) (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat# MAB12764) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat # MAB12764) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse immunoglobulin kappa light chain.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.005 - 0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5 - 2 ug/mL)
Immunohistochemistry (0.5 - 2 ug/mL)
Immunoprecipitation
Western Blot (0.1 - 0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat # MAB12764) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (kappa) (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat# MAB12764) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat # MAB12764) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — Igk-C
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