FOLH1 monoclonal antibody, clone k1H7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of LNCaP cell lysate.
Immunofluorescence
Immunofluorescence analysis of LNCaP cells. The cell was stained with FOLH1 monoclonal antibody, clone k1H7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of LNCaP cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FOLH1.
Immunogen
Recombinant protein corresponding to amino acids 117-351 of human FOLH1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LNCaP cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of LNCaP cells. The cell was stained with FOLH1 monoclonal antibody, clone k1H7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of LNCaP cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — FOLH1
Entrez GeneID
2346GeneBank Accession#
NM_001014986Protein Accession#
NP_001014986Gene Name
FOLH1
Gene Alias
FGCP, FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA, mGCP
Gene Description
folate hydrolase (prostate-specific membrane antigen) 1
Omim ID
600934Gene Ontology
HyperlinkGene Summary
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq
Other Designations
N-acetylated alpha-linked acidic dipeptidase 1|cell growth-inhibiting protein 27|folate hydrolase 1|folylpoly-gamma-glutamate carboxypeptidase|glutamate carboxylase II|glutamate carboxypeptidase II|membrane glutamate carboxypeptidase|prostate specific mem
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Interactome
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Disease
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Publication Reference
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Prostate-specific membrane antigen associates with anaphase-promoting complex and induces chromosomal instability.
Rajasekaran SA, Christiansen JJ, Schmid I, Oshima E, Ryazantsev S, Sakamoto K, Weinstein J, Rao NP, Rajasekaran AK.
Molecular Cancer Therapeutics 2008 Jul; 7(7):2142.
Application：IEM, IF, IP, Human, PC-3 cells.
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Induction of autoantibodies to syngeneic prostate-specific membrane antigen by xenogeneic vaccination.
Gregor PD, Wolchok JD, Turaga V, Latouche JB, Sadelain M, Bacich D, Heston WD, Houghton AN, Scher HI.
International Journal of Cancer 2005 Sep; 116(3):415.
Application：Flow Cyt, IHC-P, WB, Mouse, Mouse kidney, NIH/3T3 cells.
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Targeting novel antigens for prostate cancer treatment: focus on prostate-specific membrane antigen.
Slovin SF.
Expert Opinion on Therapeutic Targets 2005 Jun; 9(3):561.
Application：ELISA, Flow Cyt, IHC, WB, Human, Mouse, Bladder, Melanoma, Lung, Kidney, Serum, Pancreas, Prostate cancers, NIH/3T3 cells.
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Prostate-specific membrane antigen associates with anaphase-promoting complex and induces chromosomal instability.
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