PDZK1 monoclonal antibody, clone AT1A2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by PDZK1 monoclonal antibody, clone AT1A2 (Cat # MAB11209) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa. Lane 2: 293T.
Immunofluorescence
Immunofluorescence analysis of PDKZ1 in Hep3B cells. The cell was stained with PDZK1 monoclonal antibody, clone AT1A2 (Cat # MAB11209) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of PDZK1 in Hep3B cell line, staining at 2-5 ug for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PDZK1.
Immunogen
Recombinant protein corresponding to amino acids 1-519 of human PDZK1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by PDZK1 monoclonal antibody, clone AT1A2 (Cat # MAB11209) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa. Lane 2: 293T.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of PDKZ1 in Hep3B cells. The cell was stained with PDZK1 monoclonal antibody, clone AT1A2 (Cat # MAB11209) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of PDZK1 in Hep3B cell line, staining at 2-5 ug for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — PDZK1
Entrez GeneID
5174Protein Accession#
NP_001188254Gene Name
PDZK1
Gene Alias
CAP70, CLAMP, NHERF3, PDZD1
Gene Description
PDZ domain containing 1
Omim ID
603831Gene Ontology
HyperlinkOther Designations
C-terminal linking and modulating protein|OTTHUMP00000015572
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Interactome
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Disease
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